Purification and properties of a major isoform of β-1,3-glucanase from pearl millet seedlings

被引：0
|
作者：
Kini, KR
Vasanthi, NS
Umesh-Kumar, S
Shetty, HS [1 ]
机构：
[1] Univ Mysore, Downy Mildew Res Lab, Dept Studies Appl Bot & Biotechnol, Mysore 570006, Karnataka, India
[2] Cent Food Technol Res Inst, Mol Biol Unit, Dept Microbiol & Bioengn, Mysore 570013, Karnataka, India
来源：
PLANT SCIENCE | 2000年 / 150卷 / 02期
关键词：
beta-1,3-glucanase; pearl miller; downy mildew; host-pathogen interaction;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A major isoform of beta-1,3-glucanase from pearl miller seedlings was purified following ammonium sulfate precipitation, ion-exchange chromatography and gel filtration techniques. The enzyme had a molecular weight of 20.5 kDa on SDS-PAGE and was highly basic with a pI of 9.6. It was thermostable with a broad temperature optima for activity ranging from 37 to 70 degrees C and had an optimum pH of 5.2. Mercuric chloride and para-chloromercuric benzoate inhibited completely the enzyme while manganese chloride activated it. Antibodies raised against the purified beta-1,3-glucanase identified another protein with an apparent molecular weight of 30 kDa in western reactions. Significance of this enzyme in pearl millet-downy mildew host-pathogen interaction is discussed. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码：139 / 145
页数：7
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