A novel wound dressing material with prolonged action

The development and creation of non-toxic dressing materials exhibiting pronounced antimicrobial effect and accelerating wound-healing process without increasing pathogenic microorganisms drug resistance is an actual task of modern medicine. Aim. Evaluation of toxicological characteristics of a dressing material containing 6 % and 9 % active chlorine in a single application on the skin as well as the study of dressing material effect on the epithelization rate of the uninfected wound surface and determination of its antimicrobial and wound healing activity in vivo on a model of S. aureus ATCC 6538-infected wound. Materials and methods. An experimental study of the dressings with immobilized polymeric N-chlorosulfonamide sodium and N, N-dichlorosulfonamide was performed to evaluate the safety in use and pharmacological activity in vivo. Evaluation of the dressing material toxicological characteristics in a single application on the skin was carried out on laboratory rats and rabbits according to the generally accepted method, the wound healing effect was studied using the test by L.N. Popova and antimicrobial activity in vivo - on a model of wound infected by the insertion of a 24-hour culture of S. aureus ATCC 6538 contaminated silk thread through the skin. Results. Acute toxicity pharmacological studies of material test-samples containing 6 % and 9 % active chlorine in a single application on the skin of rats and rabbits found that these applications did not lead to deaths, as well as changes in their behavioral pattern, consumption of feed and water. The epithelization rate of uninfected wound surface in application of dressings containing both sodium N-chlorosulfonamide and N, N-dichlorosulfonamide was significantly, on average 1.5 times, faster at the end of the observational period (day 14) in comparison with the control group animals. According to the data obtained, the application of dressings containing sodium N-chlorosulfonamide on the 24-hour culture of S. aureus ATCC 6538 infected wound significantly reduced the intensity of the inflammatory process local manifestations and 4-10 times decreased the number of pathogenic staphylococci under bandages on day 8 of observation. Conclusions. Thus, a single application of native materials on the animal skin does not cause a lethal outcome. Skin application of polymeric materials on uninfected wounds stimulates the processes of natural reparation, and decreases the number of pathogenic microorganisms in wound fluid as well as shortens the healing time in staphylococcus-infected wounds.