High-throughput separations using multiplexed capillary electrophoresis with laser-induced fluorescence detection

The authors evaluated the performance of multiplexed capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) for high-throughput separation-based assays using fluorescein isothiocyanate as a model compound and fluorescein as an internal standard. They assessed performance areas, including linearity of response, precision of migration time, peak area and peak height response with and without internal standard correction, limit of detection, cross-talk between adjacent capillaries, and reproducibility of performance over time. In this article, they also discuss representative data for the application of multiplexed CE-LIF to screening enzyme inhibitors and measuring endogenous enzyme levels.