Impairment of Interleukin-17A Expression in Canine Visceral Leishmaniosis is Correlated with Reduced Interferon-γ and Inducible Nitric Oxide Synthase Expression

被引:22
|
作者
Nascimento, M. S. L. [1 ]
Albuquerque, T. D. R. [2 ]
Nascimento, A. F. S. [3 ]
Caldas, I. S. [3 ]
Do-Valle-Matta, M. A. [4 ]
Souto, J. T. [2 ]
Talvani, A. [3 ]
Bahia, M. T. [3 ]
Galvao, L. M. C. [5 ]
Camara, A. C. J. [6 ]
Guedes, P. M. M. [2 ]
机构
[1] Univ Sao Paulo, Med Sch Ribeirao Preto, Dept Biochem & Immunol, BR-14049 Ribeirao Preto, Brazil
[2] Univ Fed Rio Grande do Norte, Dept Microbiol & Parasitol, BR-59072970 Natal, RN, Brazil
[3] Univ Fed Ouro Preto, Dept Biol Sci, NUPEB, Ouro Preto, Brazil
[4] Fiocruz MS, Inst Oswaldo Cruz, Lab Cellular Ultrastruct, BR-21045900 Rio De Janeiro, Brazil
[5] Univ Fed Minas Gerais, Dept Parasitol, Belo Horizonte, MG, Brazil
[6] Univ Fed Rio Grande do Norte, Dept Clin & Toxicol Anal, BR-59072970 Natal, RN, Brazil
关键词
canine visceral leishmaniosis; inducible nitric oxide synthase; interleukin-17; liver; IMMUNE-RESPONSES; DOGS; INFECTION; SUSCEPTIBILITY; MACROPHAGES; DISEASE; PARASITISM; CHEMOKINE; PATHOLOGY; TISSUES;
D O I
10.1016/j.jcpa.2015.10.174
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Dogs are the primary urban reservoir of Leishmania infantum and play a crucial role in the transmission of this parasite to man via sandflies. The spleen and liver are the main target organs of L. infantum infection, but few studies have evaluated the immune response to this infection in the canine liver. To identify the immunological mediators involved in resistance and/or susceptibility to canine visceral leishmaniosis (CVL), we selected 21 dogs naturally infected by L. infantum and classified as asymptomatic or symptomatic. Immunological parameters were analysed and correlations with clinical signs were determined. Symptomatic dogs showed higher numbers of parasites and less leucocyte infiltration in the liver compared with asymptomatic dogs. The progression of this disease was characterized not only by the down regulation of T helper (Th) 1-related cytokines, such as interferon (IFN)-gamma and tumour necrosis factor (TNF)-alpha, but also by the down regulation of genes encoding interleukin (IL)-17A, inducible nitric oxide synthase (iNOS) and IL-10 in the spleen and liver in symptomatic dogs compared with asymptomatic dogs. Importantly, IL-17A gene transcription level was positively correlated with mRNA expression for iNOS and IFN-gamma Th1 - and Th17-related cytokines therefore appear to play a role in restricting parasite growth via iNOS activation and decrease susceptibility of dogs to CVL. (C) 2015 Elsevier Ltd. All rights reserved.
引用
收藏
页码:197 / 205
页数:9
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