The effect of homocysteine on the expression of CD36, PPARγ, and C/EBPα in adipose tissue of normal and obese mice

被引：1
|
作者：
Mentese, Ahmet [1 ]
Dogramaci, Seniz [2 ]
Demir, Selim [3 ]
Yaman, Serap Ozer [2 ]
Ince, Imran [2 ]
Altay, Diler Us [4 ]
Erdem, Mehmet [2 ]
Turane, Ibrahim [5 ]
Alver, Ahmet [2 ]
机构：
[1] Karadeniz Tech Univ, Vocat Sch Hlth Sci, Program Med Lab Tech, Trabzon, Turkey
[2] Karadeniz Tech Univ, Fac Med, Dept Med Biochem, Trabzon, Turkey
[3] Karadeniz Tech Univ, Fac Hlth Sci, Dept Nutr & Dietet, Trabzon, Turkey
[4] Ordu Univ, Ulubey Vocat Sch, Dept Chem & Chem Proc Technol, Ordu, Turkey
[5] Gumushane Univ, Fac Engn & Nat Sci, Dept Genet & Bioengn, Gumushane, Turkey
来源：
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY | 2021年 / 127卷 / 05期
关键词：
Adipose tissue; cardiovascular diseases; CD36; C; EBP alpha; hyperhomocysteinemia; obesity; PPAR gamma; DIET-INDUCED OBESITY; ADIPOCYTE DIFFERENTIATION; GENE-EXPRESSION; METABOLISM; ATHEROSCLEROSIS; ADIPOGENESIS; INFLAMMATION; STRAIN; CELLS; LIVER;
D O I：
10.1080/13813455.2019.1648517
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The purpose of this study was to investigate the effect of homocysteine (Hcy) on CD36, PPAR gamma, and C/EBP alpha gene and protein expression in adipose tissue obtained from normal and high-calorie diet obesity models. CD36, PPAR gamma, and C/EBP alpha gene expression and protein levels in adipose tissue specimens were determined using the RT-PCR and ELISA methods, respectively. Significantly increased CD36 gene expression was observed in adipose tissue from obese mice, while Hcy significantly reduced CD36 gene expression in adipose tissue from normal and obese mice. PPAR gamma and C/EBP alpha gene expression levels decreased significantly in all groups compared to the normal group. In addition, levels of both PPAR gamma and C/EBP alpha gene expression were lower with Hcy supplementation compared to their own controls. In conclusion, Hcy's reduction of CD36 gene expression in adipose tissue may be one probable factor in hyperhomocysteinemia representing an independent risk factor for cardiovascular diseases.
引用
收藏
页码：437 / 444
页数：8
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