Imaging the noncentrosymmetric structural organization of tendon with Interferometric Second Harmonic Generation microscopy

被引：26

作者：

Rivard, Maxime ^{[1
]}

Popov, Konstantin ^{[2
]}

Couture, Charles-Andre ^{[1
]}

Laliberte, Mathieu ^{[1
]}

Bertrand-Grenier, Antony ^{[1
]}

Martin, Francois ^{[1
]}

Pepin, Henri ^{[1
]}

Pfeffer, Christian P. ^{[3
]}

Brown, Cameron ^{[4
]}

Ramunno, Lora ^{[2
]}

Legare, Francois ^{[1
]}

机构：

[1] Inst Natl Rech Sci, Ctr Energie Mat Telecommun, Varennes, PQ J3X 1S2, Canada

[2] Univ Ottawa, Dept Phys, Ottawa, ON K1N 6N5, Canada

[3] Univ Munich, Dept Heart Surg, D-81377 Munich, Germany

[4] Univ Oxford, Botnar Res Ctr, NDORMS, Oxford OX1 2JD, England

来源：

JOURNAL OF BIOPHOTONICS | 2014年 / 7卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Interferometry; Second Harmonic Generation; microscopy; tendon; collagen; connective tissues; tissue imaging; nonlinear optics; CONNECTIVE-TISSUE POLARITY;

D O I：

10.1002/jbio.201300036

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the imaging of tendon with Interferometric Second Harmonic Generation microscopy. We observe that the noncentrosymmetric structural organization can be maintained along the fibrillar axis over more than 150 mu m, while in the transverse direction it is similar to 1-15 mu m. Those results are explained by modeling tendon as a heterogeneous distribution of noncentrosymmetric nano-cylinders (collagen fibrils) oriented along the fibrillar axis. The preservation of the noncentrosymmetric structural organization over multiple tens of microns reveals that tendon is made of domains in which the ratio between fibrils with positive and negative polarity is unbalanced.

引用

页码：638 / 646

页数：9

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