Research on Fluorescence Detection System of Ca2+

A new fluorescence-micro-imaging system has been built using the microfluidic chip. By using this system fluorescence ratio of calcium ion (Ca2+) in cells were measured. Cell fostering, staining and driving can be implemented on the designed microfluidic chip. Then we make use of 340nm or 380nm laser to excite the calcium ion (Ca2+) and enable it emit fluorescence. The image of fluorescence was collected and analyzed with fluorescence-micro-imaging system. The concentration of calcium ion in cells can be calculated by using the fluorescence ratio method. Based on this new fluorescence micro-imaging system, a new application domain was opened for microfluidic chip and a new detection means was developed for measuring calcium ion (Ca2+) in cells.