Molecular Characterization and Functional Analysis of Leucine Zipper Transcription Factor Like in Zebrafish (Danio rerio)

被引:4
|
作者
Wei, Qun [1 ]
Chen, Yongxia [1 ]
Gu, Yi-Feng [2 ]
Zhao, Wenhe [1 ]
机构
[1] Zhejiang Univ, Sir Run Run Shaw Hosp, Sch Med, Dept Surg Oncol,Inst Clin Med, Hangzhou, Zhejiang, Peoples R China
[2] Zhejiang Univ, Affiliated Hosp 1, State Key Lab Diag & Treatment Infect Dis,Sch Med, Collaborat Innovat Ctr Diag & Treatment Infect Di, Hangzhou, Zhejiang, Peoples R China
来源
FRONTIERS IN PHYSIOLOGY | 2019年 / 10卷
基金
中国国家自然科学基金;
关键词
LZTFL1; HH; tumor suppressor; sequence analysis; zebrafish; BARDET-BIEDL SYNDROME; LZTFL1; DEGENERATION; ACTIVATION; GENETICS; CILIA;
D O I
10.3389/fphys.2019.00801
中图分类号
Q4 [生理学];
学科分类号
071003 ;
摘要
Leucine zipper transcription factor like 1 (LZTFL1) is a member of the Bardet-Biedl syndrome gene family. LZTFL1-null mice show the phenotype of obesity, retinal degeneration, and abnormal cilia development. Functionally, LZTFL1 serves as a tumor suppressor and a negative regulator in the hedgehog signaling pathways. The biological function of mammalian LZTFL1 is partially addressed, but data on other model organisms are limited. Zebrafish (Danio rerio) is widely considered as a powerful model to understand the functions of genes implicated in obesity, disease, and cancer. In this study, LZTFL1 homologs were identified in zebrafish (zebrafish LZTFL1). The full-length cDNA of zebrafish LZTFL1 contained 897 bps encoding 298 amino acids. Zebrafish LZTFL1 displayed conserved domains of coil-coil and leucine zipper domain. PCR results showed that zebrafish LZTFL1 was widely distributed in various tissues. Western blot analysis further revealed that zebrafish LZTFL1 was detected to be ectopically expressed in HeLa cells with correct molecular weight. Fluorescence images showed as well that zebrafish LZTFL1 was localized in the cytoplasm. Furthermore, luciferase reporter assay indicated zebrafish LZTFL1 served as a negative regulator in the hedgehog signaling pathway. These data supported that zebrafish was a good model for understanding the biological roles of LZTFL1.
引用
收藏
页数:8
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