Chlorpyrifos releases norepinephrine from adult and neonatal rat brain synaptosomes

被引:38
|
作者
Dam, K [1 ]
Seidler, FJ [1 ]
Slotkin, TA [1 ]
机构
[1] Duke Univ, Med Ctr, Dept Pharmacol & Canc Biol, Durham, NC 27710 USA
来源
DEVELOPMENTAL BRAIN RESEARCH | 1999年 / 118卷 / 1-2期
关键词
chlorpyrifos; development; norepinephrine; synaptosome;
D O I
10.1016/S0165-3806(99)00139-X
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Exposure of developing animals to apparently subtoxic doses of chlorpyrifos (CPF) during a critical period of synaptogenesis has been shown to affect catecholaminergic synaptic development and neuronal activity separably from its inhibition of cholinesterase. We used rat brain synaptosome preparations to examine whether CPF has a direct effect on the release of norepinephrine (NE). Synaptosomes were preloaded with [H-3]NE in the absence of CPF and were then exposed to the compound during subsequent neurotransmitter release. There was a robust increase in release at 50 mu g/ml of CPF. The effect was not mediated through cholinergic receptors, as neither atropine nor mecamylamine interfered with the actions of CPF. Enhanced NE release was seen in synaptosomes derived from neonatal rat brain as well as adult rat brain, albeit with a smaller effect in neonates. Our results suggest that CPF interacts directly with presynaptic nerve terminals to influence neurotransmitter release; in the context of the immature brain, these effects can alter synaptic development through the trophic actions of catecholamines. (C) 1999 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:129 / 133
页数:5
相关论文
共 50 条
  • [41] PROTEIN-SYNTHESIS BY SYNAPTOSOMES FROM RAT-BRAIN
    HERNANDEZ, AG
    BIOCHEMICAL JOURNAL, 1974, 142 (01) : 7 - 17
  • [43] TRANSPORT OF CYSTEATE BY SYNAPTOSOMES ISOLATED FROM RAT-BRAIN
    WILSON, DF
    PASTUSZKO, A
    FEDERATION PROCEEDINGS, 1986, 45 (06) : 1729 - 1729
  • [44] CHARACTERIZATION OF THE GLUCOSE TRANSPORTER FROM RAT-BRAIN SYNAPTOSOMES
    BHATTACHARYYA, MV
    BRODSKY, JL
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 155 (02) : 685 - 691
  • [45] NOREPINEPHRINE IN FETAL AND NEONATAL RABBIT BRAIN
    MOTELICAHEINO, I
    ROFFI, J
    EXPERIENTIA, 1975, 31 (10): : 1166 - 1167
  • [46] Influence of ketoacidosis on rat brain synaptosomes
    Voronina, P.
    Adamovich, K.
    Adamovich, T.
    Hrynevich, S.
    Waseem, T.
    Fedorovich, S.
    ACTA PHYSIOLOGICA, 2019, 227 : 132 - 132
  • [47] EFFECT OF DIETARY LIPIDS ON NOREPINEPHRINE RELEASE IN PERFUSED NEONATAL AND ADULT-RAT HEARTS
    SEMAFUKO, WEB
    RUTLEDGE, CO
    DIXON, WR
    FEDERATION PROCEEDINGS, 1983, 42 (03) : 636 - 636
  • [48] EFFECTS OF MATERNAL ETHANOL INGESTION ON AMINE UPTAKE INTO SYNAPTOSOMES OF FETAL AND NEONATAL RAT-BRAIN
    THADANI, PV
    LAU, C
    SLOTKIN, TA
    SCHANBERG, SM
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 1977, 200 (02): : 292 - 297
  • [49] Distribution of GFAP plus astrocytes in adult and neonatal rat brain
    Taft, JR
    Vertes, RP
    Perry, GW
    INTERNATIONAL JOURNAL OF NEUROSCIENCE, 2005, 115 (09) : 1333 - 1343
  • [50] Effect of age, gender, rat strain, and dietary restriction, on norepinephrine release from cardiac synaptosomes
    Snyder, DL
    Gayheart-Walsten, PA
    Rhie, S
    Wang, WJ
    Roberts, J
    JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 1998, 53 (01): : B33 - B41