Cyclodextrins as buffer additives for the enantiomeric separation of pinacidil in capillary zone electrophoresis

被引：9
|
作者：
Wang, Z
Huang, AJ
Sun, YL
Sun, ZP
机构：
[1] BEIJING UNIV,DEPT CHEM,BEIJING 100817,PEOPLES R CHINA
[2] NATL INST CONTROL PHARMACEUT & BIOL PROD,BEIJING 100050,PEOPLES R CHINA
来源：
JOURNAL OF CHROMATOGRAPHY A | 1996年 / 749卷 / 1-2期
关键词：
buffer composition; enantiomer separation; pinacidil;
D O I：
10.1016/0021-9673(96)00431-1
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The enantiomeric separation of pinacidil was studied using alpha-cyclodextrin (alpha-CD), beta-CD, heptakis (2,6-di-O-methyl)-beta-CD (DM-beta-CD), hydroxypropyl-beta-CD (HP-beta-CD) and gamma-CD as buffer additives at acidic pH in capillary zone electrophoresis. beta-CD, HP-beta-CD and gamma-CD all showed chiral recognition on the compound, with HP-beta-CD giving the highest chiral selectivity. Buffer concentration and HP-beta-CD concentration were optimized for the separation. The separation was improved by the addition of hydroxypropyl cellulose (HPC) to the buffer. A complete separation of pinacidil enantiomers was achieved by using 100 mM Tris-H3PO4 buffer (pH 2.3) containing 9 mM HP-beta-CD and 0.05% HPC.
引用
收藏
页码：300 / 303
页数：4
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