Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid

被引：32

作者：

Stegurova, Lucie ^{[1
]}

Draberova, Eduarda ^{[2
]}

Bartos, Ales ^{[3
,4
,5
]}

Draber, Pavel ^{[2
]}

Ripova, Daniela ^{[3
,4
]}

Draber, Petr ^{[1
]}

机构：

[1] Acad Sci Czech Republic, Inst Mol Genet, Lab Signal Transduct, CZ-14220 Prague 4, Czech Republic

[2] Acad Sci Czech Republic, Inst Mol Genet, Lab Biol Cytoskeleton, CZ-14220 Prague 4, Czech Republic

[3] Natl Inst Mental Hlth, Prague Psychiat Ctr, Lab Biochem & Brain Pathophysiol, CZ-18103 Prague 8, Czech Republic

[4] Natl Inst Mental Hlth, Prague Psychiat Ctr, AD Ctr, CZ-18103 Prague 8, Czech Republic

[5] Charles Univ Prague, Univ Hosp Kralovske Vinohrady, Fac Med 3, Dept Neurol, CZ-10034 Prague 10, Czech Republic

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2014年 / 406卷

关键词：

Gold nanoparticles; PCR; ELISA; Tau protein; ALZHEIMERS-DISEASE;

D O I：

10.1016/j.jim.2014.03.007

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Tau protein in cerebrospinal fluid (CSF) is an important biomarker of Alzheimer's disease and some other brain diseases. Enzyme-linked immunosorbent assays (ELISAs) have been mostly used for quantification of tau and other biomarkers in CSF. However, these assays do not have sufficient sensitivity and dynamic range. In this study we tested the suitability of gold nanoparticles functionalized with tau-specific monoclonal antibody and oligonucleotide template for immunopolymerase chain reaction (Nano-iPCR) quantification of tau protein in human CSF samples and compared it with ELISA, either commercial or newly developed with tyramide signal amplification. Our data indicate that Nano-iPCR is superior in sensitivity and detection range to ELISA in tau protein detection. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：137 / 142

页数：6

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