A continuous-flow high-throughput microfluidic device for airborne bacteria PCR detection

被引：49
|
作者：
Jiang, Xiran [1 ]
Jing, Wenwen [1 ,2 ]
Zheng, Lulu [1 ]
Liu, Sixiu [1 ]
Wu, Wenjuan [3 ]
Sui, Guodong [1 ]
机构：
[1] Fudan Univ, Inst Biomed Sci, Dept Environm Sci & Engn, Shanghai Key Lab Atmospher Particle Pollut Preven, Shanghai 200433, Peoples R China
[2] Fudan Univ, Sch Basic Med Sci, Dept Med Microbiol & Parasitol, Shanghai 200032, Peoples R China
[3] Tongji Univ, Shanghai East Hosp, Dept Lab Med, Shanghai 200120, Peoples R China
来源：
LAB ON A CHIP | 2014年 / 14卷 / 04期
关键词：
NUCLEIC-ACID AMPLIFICATION;
D O I：
10.1039/c3lc50977j
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Rapid analysis of airborne pathogens plays a critical role in early warning of spreading infectious diseases, which is essential for public health and disease prevention. Herein we report an integrated microfluidic device that can perform airborne pathogen capture, enrichment and continuous-flow high-throughput gene analysis. The device was validated by six frequently encountered bacteria and shows great potential in environmental analysis and for public health protection.
引用
收藏
页码：671 / 676
页数：6
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