Identification of transcripts related to high egg production in the chicken hypothalamus and pituitary gland

被引:37
|
作者
Shiue, Yow-Ling
Chen, Lih-Ren
Chen, Chih-Feng
Chen, Yi-Ling
Ju, Jhy-Phen
Chao, Ching-Hsien
Lin, Yuan-Ping
Kuo, Yu-Ming
Tang, Pin-Chi
Lee, Yen-Pai [1 ]
机构
[1] Natl Chung Hsing Univ, Dept Anim Sci, Taichung 40227, Taiwan
[2] Natl Sun Yat Sen Univ, Inst Biomed Sci, Kaohsiung 80424, Taiwan
[3] Council Agr, Div Physiol, Livestock Res Inst, Tainan, Taiwan
[4] Natl Chiayi Univ, Dept Anim Sci, Chiayi, Taiwan
[5] Natl Cheng Kung Univ, Coll Med, Dept Cell Biol & Anat, Tainan 70101, Taiwan
关键词
chickens; egg production; hypothalamus; pituitary gland; transcriptomics;
D O I
10.1016/j.theriogenology.2006.03.037
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
To identify transcripts related to high egg production expressed specifically in the hypothalamus and pituitary gland of the chicken, two subtracted cDNA libraries were constructed. Two divergently selected strains of Taiwan Country Chickens (TCCs), B (sire line) and L2 (dam line) were used; they had originated from a single population and were further subjected (since 1982) to selection for egg production to 40 wk of age and body weight/comb size, respectively. A total of 324 and 370 clones were identified from the L2-B (L2-subtract-B) and the B-L2 subtracted cDNA libraries, respectively. After sequencing and annotation, 175 and 136 transcripts that represented 53 known and 65 unknown non-redundant sequences were characterized in the L2-B subtracted cDNA library. Quantitative reverse-transcription (RT)-PCR was used to screen the mRNA expression levels of 32 randomly selected transcripts in another 78 laying hens from five different strains. These strains included the two original strains (13 and L2) used to construct the subtracted cDNA libraries and an additional three commercial strains, i.e., Black- and Red-feather TCCs and Single-Comb White Leghorn (WL) layer. The mRNA expression levels of 16 transcripts were significantly higher in the L2 than in the B strain, whereas the mRNA expression levels of nine transcripts, BDH, NCAM1, PCDHA@, PGDS, PLAG1, PRL, SAR1A, SCG2 and STMN2, were significantly higher in two high egg production strains, L2 and Single-Comb WL; this indicated their usefulness as molecular markers of high egg production. (c) 2006 Elsevier Inc. All rights reserved.
引用
收藏
页码:1274 / 1283
页数:10
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