Borrelia burgdorferi chemotaxis toward tick protein Salp12 contributes to acquisition

被引:20
|
作者
Murfin, Kristen E. [1 ]
Kleinbard, Ruby [1 ,2 ]
Aydin, Merve [1 ,3 ]
Salazar, Samuel A. [1 ,4 ]
Fikrig, Erol [1 ,5 ]
机构
[1] Yale Univ, Sch Med, Dept Internal Med, Infect Dis Sect, 333 Cedar St, New Haven, CT 06519 USA
[2] Emory Univ, Sch Med, Dept Pediat, Ctr AIDS Res,Lab Biochem Pharmacol, Atlanta, GA 30322 USA
[3] KTO Karatay Univ, Dept Med Microbiol, Sch Med, TR-42020 Konya, Turkey
[4] Florida Int Univ, Dept Biol Sci, Miami, FL 33199 USA
[5] Howard Hughes Med Inst, Chevy Chase, MD 20815 USA
关键词
Borrelia burgdorferi sensu stricto; Chemotaxis; Acquisition; Lxodes scapularis; Tick; Lyme disease; LYME-DISEASE SPIROCHETE; FLOW-CYTOMETRY; MIGRATION; TRANSMISSION; INFECTION; DOMAIN; AGENT;
D O I
10.1016/j.ttbdis.2019.06.002
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Lyme disease is a common tick-borne infection caused by the spirochete Borrelia burgdorferi sensu stricto (s.s.). B. burgdorferi s.s. may utilize chemotaxis, the directional migration towards or away from a chemical stimulus, for transmission, acquisition, and infection. However, the specific signals recognized by the spirochete for these events have not been defined. In this study, we identify an Ixodes scapularis salivary gland protein, Salp12, that is a chemoattractant for the spirochete. We demonstrate that Salp12 is expressed in the I. scapularis salivary glands and midgut and expression is not impacted by B. burgdorferi s.s. infection. Knockdown of Salp12 in the salivary glands or passive immunization against Salp12 reduces acquisition of the spirochete by ticks but acquisition is not completely prevented. Knockdown does not impact transmission of B. burgdorferi s.s. This work suggests a new role for chemotaxis in acquisition of the spirochete and suggests that recognition of Salp12 contributes to this phenomenon.
引用
收藏
页码:1124 / 1134
页数:11
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