FATTY ACIDS DETERMINATION IN TROUT PLASMA AND MEAT BY GC-MS

被引：0

作者：

Suvar, Sonia ^{[1
]}

Horj, Elena ^{[1
]}

Podea, Paula ^{[2
]}

Iordache, Andreea ^{[3
]}

Cocan, Daniel ^{[4
]}

Culea, Monica ^{[1
]}

机构：

[1] Univ Babes Bolyai, Fac Phys, 1 Kogalniceanu Str, Cluj Napoca 400084, Romania

[2] Univ Babes Bolyai, Fac Chem & Chem Engn, RO-400084 Cluj Napoca, Romania

[3] Natl R&D Inst Cryogen & Isotop Technol, Ramnicu Valcea 240050, Romania

[4] Univ Agr Sci & Vet Med, Cluj Napoca 400372, Romania

来源：

STUDIA UNIVERSITATIS BABES-BOLYAI CHEMIA | 2015年 / 60卷 / 02期

关键词：

DHA; EPA; essential fatty acids; FAME; GC-MS; SFA; UFA; MASS-SPECTROMETRY; FISH; WILD;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The aim of this study was to develop a simple and reliable GC-MS method to compare the fatty acids in trout plasma and meat. The lipids were extracted from 0.5 mL of plasma and 1 g of meat using chloroform:methanol 1:2 (v:v) and then derivatized into fatty acids methyl esters (FAMEs) by esterification with methanol:acetyl chloride 4:1 (v:v). For FAMEs quantitation, undecaenoic acid (C11:1) was used as internal standard. High proportions of unsaturated fatty acids (UFAs) were found both in plasma and in meat samples. The highest proportion of UFAs in meat samples (53%) was represented by the omega-3 fatty acids.

引用

页码：109 / 116

页数：8

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