Flagellin and F4 fimbriae have opposite effects on biofilm formation and quorum sensing in F4ac+enterotoxigenic Escherichia coli

被引：39
|
作者：
Zhou, Mingxu [1 ,2 ]
Guo, Zhiyan [1 ,2 ]
Yang, Yang [1 ,2 ]
Duan, Qiangde [1 ,3 ]
Zhang, Qi [1 ,2 ]
Yao, Fenghua [1 ,2 ]
Zhu, Jun [1 ,2 ]
Zhang, Xinjun [1 ,2 ]
Hardwidge, Philip R. [4 ]
Zhu, Guoqiang [1 ,2 ]
机构：
[1] Yangzhou Univ, Coll Vet Med, Yangzhou 225009, Peoples R China
[2] Jiangsu Co Innovat Ctr Important Anim Infect Dis, Yangzhou 225009, Peoples R China
[3] Weinan Vocat & Tech Coll, Coll Agr, Weinan 714000, Peoples R China
[4] Kansas State Univ, Coll Vet Med, Manhattan, KS 66506 USA
来源：
VETERINARY MICROBIOLOGY | 2014年 / 168卷 / 01期
基金：
美国国家科学基金会;
关键词：
Flagellin; F4; fimbriae; Biofilm formation; Quorum sensing; IN-VITRO; SALMONELLA-TYPHIMURIUM; EXPRESSION; VIRULENCE; LUXS; MOTILITY; GENES; CONTRIBUTES; INFECTION; CELLS;
D O I：
10.1016/j.vetmic.2013.10.014
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Bacteria that form biofilms are often highly resistant to antibiotics and are capable of evading the host immune system. To evaluate the role of flagellin and F4 fimbriae on biofilm formation by enterotoxigenic Escherichia coli (ETEC), we deleted the fliC (encoding the major flagellin protein) and/or the faeG (encoding the major subunit of F4 fimbriae) genes from ETEC C83902. Biofilm formation was reduced in the fliC mutant but increased in the faeG mutant, as compared with the wild-type strain. The expression of AI-2 quorum sensing associated genes was regulated in the fliC and faeG mutants, consistent with the biofilm formation of these strains. But, deleting fliC and/or faeG also inhibited AI-2 quorum sensing activity. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码：148 / 153
页数：6
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