A colorimetric sandwich-type assay for sensitive thrombin detection based on enzyme-linked aptamer assay

被引：30
|
作者：
Park, Jun Hee
Cho, Yea Seul
Kang, Sungmuk
Lee, Eun Jeong
Lee, Gwan-Ho
Hah, Sang Soo [1 ]
机构：
[1] Kyung Hee Univ, Dept Chem, Seoul 130701, South Korea
来源：
ANALYTICAL BIOCHEMISTRY | 2014年 / 462卷
基金：
新加坡国家研究基金会;
关键词：
Aptamer; Thrombin; Signal amplification; ELISA; BINDING APTAMER; MAGNETIC BEADS; DNA; NANOPARTICLES; BIOCHIP; BIOLOGY; SENSOR;
D O I：
10.1016/j.ab.2014.05.015
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A colorimetric sandwich-type assay based on enzyme-linked aptamer assay has been developed for the fast and sensitive detection of as low as 25 fM of thrombin with high linearity. Aptamer-immobilized glass was used to capture the target analyte, whereas a second aptamer, functionalized with horseradish peroxidase (HRP), was employed for the conventional 3,5,3',5'-tetramethylbenzidine (TMB)-based colorimetric detection. Without the troublesome antibody requirement of the conventional enzyme-linked immunosorbent assay (ELISA), as low as 25 fM of thrombin could be rapidly and reproducibly detected. This assay has superior, or at least equal, recovery and accuracy to that of conventional antibody-based ELISA. (C) 2014 Elsevier Inc. All rights reserved.
引用
收藏
页码：10 / 12
页数：3
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