Lack of the α1,3-Fucosyltransferase Gene (Osfuct) Affects Anther Development and Pollen Viability in Rice

被引:12
|
作者
Sim, Joon-Soo [1 ]
Kesawat, Mahipal Singh [1 ]
Kumar, Manu [2 ]
Kim, Su-Yeon [1 ]
Mani, Vimalraj [1 ]
Subramanian, Parthiban [1 ]
Park, Soyoung [1 ]
Lee, Chang-Muk [1 ]
Kim, Seong-Ryong [2 ]
Hahn, Bum-Soo [1 ]
机构
[1] Rural Dev Adm, Dept Agr Biotechnol, Metab Engn Div, Natl Inst Agr Sci, Jeonju 54874, South Korea
[2] Sogang Univ, Dept Life Sci, Seoul 121742, South Korea
关键词
alpha 1,3-fucosyltransferase; anther; development; pollen; microarray; N-glycan; viability; OLIGOSACCHARYLTRANSFERASE COMPLEX SUBUNIT; DNA INSERTIONAL MUTAGENESIS; N-GLYCOSYLATION; ARABIDOPSIS; PROTEINS; OVEREXPRESSION; EXPRESSION; TOLERANCE; DEFECTS; HOMOLOG;
D O I
10.3390/ijms19041225
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
N-linked glycosylation is one of the key post-translational modifications. alpha 1,3-Fucosyltransferase (OsFucT) is responsible for transferring alpha 1,3-linked fucose residues to the glycoprotein N-glycan in plants. We characterized an Osfuct mutant that displayed pleiotropic developmental defects, such as impaired anther and pollen development, diminished growth, shorter plant height, fewer tillers, and shorter panicle length and internodes under field conditions. In addition, the anthers were curved, the pollen grains were shriveled, and pollen viability and pollen number per anther decreased dramatically in the mutant. Matrix-assisted laser desorption/ionization time-of-flight analyses of the N-glycans revealed that alpha 1,3-fucose was lacking in the N-glycan structure of the mutant. Mutant complementation revealed that the phenotype was caused by loss of Osfuct function. Transcriptome profiling also showed that several genes essential for plant developmental processes were significantly altered in the mutant, including protein kinases, transcription factors, genes involved in metabolism, genes related to protein synthesis, and hypothetical proteins. Moreover, the mutant exhibited sensitivity to an increased concentration of salt. This study facilitates a further understanding of the function of genes mediating N-glycan modification and anther and pollen development in rice.
引用
收藏
页数:22
相关论文
共 50 条
  • [31] LIGHT MICROSCOPIC OBSERVATIONS ON POLLEN AND ANTHER DEVELOPMENT IN RICE (ORYZA-SATIVA L) .1. STAGES FROM POLLEN MOTHER CELLS TO TETRADS
    WADA, T
    OGAWA, K
    ITO, T
    SUZUKI, H
    TAKEOKA, Y
    JAPANESE JOURNAL OF CROP SCIENCE, 1990, 59 (04) : 769 - 777
  • [32] Normal embryonic and germ cell development in mice lacking α1,3-fucosyltransferase IX (Fut9) which show disappearance of stage-specific embryonic antigen 1
    Kudo, T
    Kaneko, M
    Iwasaki, H
    Togayachi, A
    Nishihara, S
    Abe, K
    Narimatsu, H
    MOLECULAR AND CELLULAR BIOLOGY, 2004, 24 (10) : 4221 - 4228
  • [34] Characterization of EBV-transformed B-cells established from an individual homozygously mutated (G329A) in the FUT7 α1,3-fucosyltransferase gene
    Bengtson, P
    Zetterberg, H
    Mellberg, T
    Påhlsson, P
    Larson, G
    SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 2005, 62 (03) : 251 - 258
  • [35] Transfection of the c-erbB2/neu gene upregulates the expression of sialyl Lewis X, α1,3-fucosyltransferase VII, and metastatic potential in a human hepatocarcinoma cell line
    Liu, F
    Qi, HL
    Zhang, Y
    Zhang, XY
    Chen, HL
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (12): : 3501 - 3512
  • [36] CaMF2, an anther-specific lipid transfer protein (LTP) gene, affects pollen development in Capsicum annuum L.
    Chen, Changming
    Chen, Guoju
    Hao, Xuefeng
    Cao, Bihao
    Chen, Qinghua
    Liu, Shaoqun
    Lei, Jianjun
    PLANT SCIENCE, 2011, 181 (04) : 439 - 448
  • [37] Restriction of high CD15 expression to a subset of rat cerebellar astroglial cells can be overcome by transduction with adenoviral vectors expressing the rat α1,3-fucosyltransferase IV gene
    Baboval, T
    Crandall, JE
    Kinnally, E
    Chou, DKH
    Smith, FI
    GLIA, 2000, 31 (02) : 144 - 154
  • [38] Inactivation of the β (1,2)-xylosyltransferase and the α (1,3)-fucosyltransferase gene in rice (Oryza sativa) by multiplex CRISPR/Cas9 strategy
    Jung, Jae-Wan
    Shin, Jun-Hye
    Lee, Won-Kyung
    Begum, Hilal
    Min, Chan-Hong
    Jang, Mi-Hwa
    Oh, Han-Bin
    Yang, Moon-Sik
    Kim, Seong-Ryong
    PLANT CELL REPORTS, 2021, 40 (06) : 1025 - 1035
  • [39] Polymorphonuclear leukocytes from individuals carrying the G329A mutation in the α1,3-fucosyltransferase VII gene (FUT7) roll on E- and P-selectins
    Bengtson, P
    Lundblad, A
    Larson, G
    Påhlsson, P
    JOURNAL OF IMMUNOLOGY, 2002, 169 (07): : 3940 - 3946
  • [40] COLLAPSED ABNORMAL POLLEN1 Gene Encoding the Arabinokinase-Like Protein Is Involved in Pollen Development in Rice
    Ueda, Kenji
    Yoshimura, Fumiaki
    Miyao, Akio
    Hirochika, Hirohiko
    Nonomura, Ken-Ichi
    Wabiko, Hiroetsu
    PLANT PHYSIOLOGY, 2013, 162 (02) : 858 - 871