Evaluation of antibiotic sensitivity of Chlamydia trachomatis using RT-PCR

被引：1
|
作者：
Misyurina, OY
Shipitsina, EV
Parfenova, TM
Lazarev, VN
Savicheva, AM
Govorun, VM
机构：
[1] Inst Physicochem Med, Moscow, Russia
[2] Russian Acad Med Sci, DO Ott Inst Obstet & Gynecol, St Petersburg, Russia
来源：
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE | 2002年 / 133卷 / 03期
关键词：
Chlamydia trachomatis; RT-PCR; antibiotics; omp3B; 16S rRN 4;
D O I：
10.1023/A:1015815607609
中图分类号：
R-3 [医学研究方法]; R3 [基础医学];
学科分类号：
1001 ;
摘要：
The sensitivity of 11 clinical strains of Chlamydia trachomatis to azithromycin, ofloxacin, doxycycline, and erythromycin was evaluated. The minimum inhibiting concentrations of all antibiotics for 90% strains, determined by PCR with reverse transcription of omp3B gene RNA (GenBank U68443) corresponded to, and those with reverse transcription of 16S rRNA gene RNA (GenBank X54451) far surpassed the minimum bactericidal concentrations for 90% strains determined by direct immuno fluorescence with monoclonal antibodies to the major outer membrane protein.
引用
收藏
页码：308 / 310
页数：3
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