An electrochemical sensor concept for the detection of bacterial virulence factors from Staphylococcus aureus and Pseudomonas aeruginosa

被引：24

作者：

Thet, Naing Tun ^{[1
]}

Jenkins, A. Toby A. ^{[1
]}

机构：

[1] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2015年 / 59卷

基金：

英国工程与自然科学研究理事会;

关键词：

Staphylococcus aureus; Pseudomonas aeruginosa; Lipid vesicles; Potassium ferricyanide; Rhamnolipid; Delta toxin; IMPEDANCE SPECTROSCOPY; MEMBRANES; BIOSENSOR;

D O I：

10.1016/j.elecom.2015.01.001

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

This work presents an electrochemical sensor concept for bacterial toxin detection using biomimetic lipid vesicles containing potassium ferricyanide. The toxin mediated release of redox couples from the vesicles was quantified by measuring the redox current on screen printed electrodes, and the detection limits to rhamnolipid, delta toxin and bacterial supernatants from clinical wound pathogens were examined. Overall detection limits of both redox and carboxyfluorescein containing vesicles were one order of magnitude lower than the cytotoxic dose of studied toxins to T lymphocytes. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：104 / 108

页数：5

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