Synthesis and incorporation of 5'-amino- and 5'-mercapto-5'-deoxy-2'-O-methyl nucleosides into hammerhead ribozymes

被引：2

作者：

MatulicAdamic, J ^{[1
]}

Haeberli, P ^{[1
]}

DiRenzo, AB ^{[1
]}

Mokler, VR ^{[1
]}

Maloney, L ^{[1
]}

Beigelman, L ^{[1
]}

Usman, N ^{[1
]}

Wincott, FE ^{[1
]}

机构：

[1] RIBOZYME PHARMACEUT INC,DEPT OLIGONUCLEOTIDE CHEM,BOULDER,CO 80301

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 10-11期

关键词：

D O I：

10.1080/07328319708002545

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel 5'-amino-5'-deoxy-2'-O-methyl uridine, guanosine and adenosine 3'-O-phosphoramidites 5, 11, and 20, as well as protected 5'-mercapto-5'-deoxy-2'-O-methyl uridine 3'-O-phosyhoramidite 23 were synthesized from 2'-O-methyl nucleosides. These analogs were incorporated at the 5'-ends of hammerhead ribozymes to evaluate achiral bridging 5'-N-phosphoramidates and 5'-S-phosphorothioates as alternatives for nonbridging phosphorothioates commonly used for end stabilization against nucleases. Oligonucleotide synthesis and deprotection conditions were optimized for better yields of these modified ribozymes.

引用

页码：1933 / 1950

页数：18

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