Characterization of Green Fluorescent Protein Using Aqueous Two-Phase Systems

被引：0

作者：

Umakoshi, Hiroshi ^{[1
]}

Nishida, Masato ^{[1
]}

Suga, Keishi ^{[1
]}

Bui, Huong Thi ^{[1
]}

Shimanouchi, Toshinori ^{[1
]}

Kuboi, Ryoichi ^{[1
]}

机构：

[1] Osaka Univ, Div Chem Engn, Grad Sch Engn Sci, Osaka 5608531, Japan

来源：

SOLVENT EXTRACTION RESEARCH AND DEVELOPMENT-JAPAN | 2009年 / 16卷

基金：

日本学术振兴会;

关键词：

FRAGMENTED SUPEROXIDE-DISMUTASE; LIPOSOME MEMBRANE; LIPID-BILAYERS; SURFACE; MUTANT;

D O I：

暂无

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The surface net hydrophobicity and local hydrophobicity of green fluorescent protein was evaluated by using the aqueous two-phase partitioning method. The surface net hydrophobicity of GFP allowed it to be classified as a hydrophobic protein although the local hydrophobicity was low. The local hydrophobicity of the GFP was increased by a pH shift to acidic conditions and was maximal at pH 4. The surface net hydrophobicity increased at acidic conditions less than pH 4. The conformational change of GFP at acidic pH is discussed based on the above results.

引用

页码：145 / 150

页数：6

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