Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors

被引：18

作者：

Kolesar, Jill ^{[1
,2
]}

Huang, Wei ^{[1
,3
]}

Eickhoff, Jens ^{[1
,4
]}

Hahn, Kristine ^{[1
,5
]}

Alberti, Dona ^{[1
,5
]}

Attia, Steven ^{[1
,5
]}

Schelman, William ^{[1
,5
]}

Holen, Kyle ^{[1
,5
]}

Traynor, Anne ^{[1
,5
]}

Ivy, Percy ^{[6
]}

Wilding, George ^{[1
,5
]}

机构：

[1] Univ Wisconsin, Paul P Carbone Comprehens Canc Ctr, Madison, WI 53792 USA

[2] Univ Wisconsin, Sch Pharm, Madison, WI 53792 USA

[3] Univ Wisconsin, Sch Med & Publ Hlth, Dept Pathol, Madison, WI 53792 USA

[4] Univ Wisconsin, Sch Med & Publ Hlth, Dept Biostat, Madison, WI 53792 USA

[5] Univ Wisconsin, Sch Med & Publ Hlth, Dept Med, Madison, WI 53792 USA

[6] NCI, Canc Therapy Evaluat Program, Bethesda, MD 20892 USA

来源：

CANCER CHEMOTHERAPY AND PHARMACOLOGY | 2009年 / 64卷 / 01期

关键词：

Triapine (R); 3-Aminopyridine-2-carboxaldehyde thiosemicarbazone; Ribonucleotide reductase; Automated quantitative immunohistochemistry (AQUA); Quantitative real time PCR; PHASE-II; CELL-PROLIFERATION; R2; COMPONENT; IN-VITRO; CANCER; OVEREXPRESSION; GEMCITABINE; TRANSFORMATION; RESISTANCE; INCREASES;

D O I：

10.1007/s00280-008-0845-0

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP. Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA). Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 +/- A 0.94 SD, vs 0.41 +/- A 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 +/- A 1.49 SD, vs 0.29 +/- A 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12). RRM2 gene and protein expression varies by tumor type.

引用

页码：79 / 86

页数：8

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