Visualization of intracellular Ca2+ dynamics with Third Harmonic Generation microscopy

被引:0
|
作者
Barille, R [1 ]
Rivet, S [1 ]
Canioni, L [1 ]
Sarger, L [1 ]
Vacher, P [1 ]
Voisin, PE [1 ]
机构
[1] CNRS, UMR 5798, Ctr Phys Mol & Ondes Hertziennes, F-33405 Talence, France
关键词
Third Harmonic Generation; Laser scanning microscopy; nonlinear imaging; calcium; medical and biological imaging; three-dimensional microscopy;
D O I
暂无
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Measurements by Laser scanning Third Harmonic Generation microscopy of Ca2+ dynamic release from internal stores and/or calcium influx in biological cells is presented. The Ca2+ signal consists of a transient increase in the intracellular concentration. A good correlation is found between these measurements and measurements done with microspectrofluorometry.
引用
收藏
页码:228 / 234
页数:7
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