An HRM Assay to Differentiate Sheeppox Virus Vaccine Strains from Sheeppox Virus Field Isolates and other Capripoxvirus Species

被引：29

作者：

Chibssa, Tesfaye Rufael ^{[1
,2
,3
]}

Settypalli, Tirumala Bharani K. ^{[1
]}

Berguido, Francisco J. ^{[1
]}

Grabherr, Reingard ^{[2
]}

Loitsch, Angelika ^{[4
]}

Tuppurainen, Eeva

Nwankpa, Nick ^{[5
]}

Tounkara, Karim ^{[5
]}

Madani, Hafsa ^{[6
]}

Omani, Amel ^{[6
]}

Diop, Mariane ^{[7
]}

Cattoli, Giovanni ^{[1
]}

Diallo, Adama ^{[7
,8
]}

Lamien, Charles Euloge ^{[1
]}

机构：

[1] IAEA, Joint FAO IAEA Agr & Biotechnol Lab, Div Nucl Tech Food & Agr, Dept Nucl Sci & Applicat,Anim Prod & Hlth Lab, Wagramer Str 5,POB 100, A-1400 Vienna, Austria

[2] Univ Nat Resources & Life Sci BOKU, Inst Biotechnol, Muthgasse 18, A-1190 Vienna, Austria

[3] NAHDIC, POB 04, Sebeta, Ethiopia

[4] Austrian Agcy Hlth & Food Safety AGES, Inst Vet Dis Control, Modling, Austria

[5] African Union Pan African Vet Vaccine Ctr AU PANV, POB 1746, Debre Ziet, Ethiopia

[6] Inst Natl Med Vet, Lab Cent Vet Alger, Algiers, Algeria

[7] ISRA, Lab Natl Elevage & Rech Vet, BP 2057 Dakar Hann, Dakar, Senegal

[8] INRA, CIRAD, UMR, ASTRE, F-24 Montpellier 05, France

来源：

SCIENTIFIC REPORTS | 2019年 / 9卷 / 1期

关键词：

LUMPY SKIN-DISEASE; RESOLUTION MELTING ANALYSIS; THREAT;

D O I：

10.1038/s41598-019-43158-x

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Sheep poxvirus (SPPV), goat poxvirus (GTPV) and lumpy skin disease virus (LSDV) affect small ruminants and cattle causing sheeppox (SPP), goatpox (GTP) and lumpy skin disease (LSD) respectively. In endemic areas, vaccination with live attenuated vaccines derived from SPPV, GTPV or LSDV provides protection from SPP and GTP. As live poxviruses may cause adverse reactions in vaccinated animals, it is imperative to develop new diagnostic tools for the differentiation of SPPV field strains from attenuated vaccine strains. Within the capripoxvirus (CaPV) homolog of the variola virus B22R gene, we identified a unique region in SPPV vaccines with two deletions of 21 and 27 nucleotides and developed a High-Resolution Melting (HRM)-based assay. The HRM assay produces four distinct melting peaks, enabling the differentiation between SPPV vaccines, SPPV field isolates, GTPV and LSDV. This HRM assay is sensitive, specific, and provides a cost-effective means for the detection and classification of CaPVs and the differentiation of SPPV vaccines from SPPV field isolates.

引用

页数：9

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