Characterization of Human Skeletal Muscle Biopsy Samples Using Shotgun Proteomics

被引：59
|
作者：
Parker, Kenneth C. [1 ,2 ,3 ]
Walsh, Ronan J. [2 ,3 ]
Salajegheh, Mohammad [2 ,3 ]
Amato, Anthony A. [3 ]
Krastins, Bryan [1 ]
Sarracino, David A. [1 ]
Greenberg, Steven A. [2 ,3 ]
机构：
[1] Harvard Partners Ctr Genet & Genom, Cambridge, MA USA
[2] Harvard Univ, Brigham & Womens Hosp, Dept Neurol, Sch Med, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Childrens Hosp Boston, Informat Program, Boston, MA USA
来源：
JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 07期
关键词：
muscle; biopsy; shotgun; abundance; protein correlation profiling; sarcomere; workflow comparison; COMPLEX; PROTEINS; TANDEM; DEPTH; FORM;
D O I：
10.1021/pr800873q
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We characterized the human muscle proteome by studying muscle biopsy specimens through four different workflows, using 1 or 2D peptide separation, SDS gels, or differential solubilization. By performing MS/MS analyses of 178 4-h LC separations derived from 31 patients, we identified more than 2000 proteins, and determined how 370 very abundant proteins behave upon differential solubilization. The resulting semiquantitative database should serve as a resource for muscle biochemistry.
引用
收藏
页码：3265 / 3277
页数：13
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