New changes in the plasma-membrane-associated proteome of rice roots under salt stress

被引:118
|
作者
Cheng, Yanwei [1 ]
Qi, Yaocheng [1 ]
Zhu, Qian [1 ]
Chen, Xi [1 ]
Wang, Ning [1 ]
Zhao, Xin [1 ]
Chen, Haiyan [1 ]
Cui, Xiangju [1 ]
Xu, Langlai [1 ]
Zhang, Wei [1 ]
机构
[1] Nanjing Agr Univ, Coll Life Sci, Dept Biochem & Mol Biol, Nanjing 210095, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
Leucine-rich-repeat receptor-like protein kinase; Oryza sativa; Plasma membrane; Proteomics; Salt stress; ABSCISIC-ACID; RESPONSIVE PROTEINS; SALINITY STRESS; GENE-EXPRESSION; ARABIDOPSIS; PLANT; TOLERANCE; COLD; KINASE; IDENTIFICATION;
D O I
10.1002/pmic.200800340
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
To gain a better understanding of salt stress responses in plants, we used a proteomic approach to investigate changes in rice (Oryza sativa) root plasma-membrane-associated proteins following treatment with 150 mmol/L NaCl. With or without a 48h salt treatment, plasma membrane fractions from root tip cells of a salt-sensitive rice cultivar, Wuyunjing 8, were purified by PEG aqueous two-phase partitioning, and plasma-membrane-associated proteins were separated by IEF/SDS-PAGE using an optimized rehydration buffer. Comparative analysis of three independent biological replicates revealed that the expressions of 18 proteins changed by more than 1.5-fold in response to salt stress. Of these proteins, nine were up-regulated and nine were down-regulated. MS analysis indicated that most of these membrane-associated proteins are involved in important physiological processes such as membrane stabilization, ion homeostasis, and signal transduction. In addition, a new leucine-rich-repeat type receptor-like protein kinase, OsRPK1, was identified as a salt-responding protein. Immuno-blots indicated that OsRPK1 is also induced by cold, drought, and abscisic acid. Using immuno-histochemical techniques, we determined that the expression of OsRPK1 was localized in the plasma membrane of cortex cells in roots. The results suggest that different rice cultivars might have different salt stress response mechanisms.
引用
收藏
页码:3100 / 3114
页数:15
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