The effects of heat shock and acclimation temperature on hsp70 and hsp30 mRNA expression in rainbow trout:: in vivo and in vitro comparisons

Heat shock (25 degrees C) of 10 degrees C-acclimated rainbow trout Oncorhynchus mykiss led to increases in heat shock protein 70 (hsp70) mRNA in blood, brain, heart, liver, red and white muscle, with levels in blood being amongst the highest. Hsp30 mRNA also increased with heat shock in all tissues with the exception of blood. When rainbow trout blood was heat shocked in vitro. both hsp70 and hsp30 mRNA increased significantly. In addition, these in vitro experiments demonstrated that blood from fish acclimated to 17 degrees C water had a lower hsp70 mRNA heat shock induction temperature than did 5 degrees C acclimated fish (20 v. 25 degrees C). The hsp30 mRNA induction temperature (25 degrees C), however, was unaffected by thermal acclimation. While increases in hsp70 mRNA levels in blood may serve as an early indicator of temperature stress in fish, tissue type, thermal history and the particular family of hsp must be considered when evaluating stress by these molecular means. (C) 2000 The Fisheries Society of the British Isles.