Production and evaluation of monoclonal antibodies for alphafetoprotein

被引：1
|
作者：
Abdel-Ghany, I. Y. [1 ]
El-Bayoumy, A. S. [1 ]
Elmouhty, N. R. A. [1 ]
Shafik, H. M. [1 ]
机构：
[1] Atom Energy Author, Hot Labs Ctr, Labeled Cpds Dept, Cairo, Egypt
来源：
JOURNAL OF RADIOANALYTICAL AND NUCLEAR CHEMISTRY | 2009年 / 280卷 / 03期
关键词：
TRACE IODINATION; THYROGLOBULIN; PROTEINS;
D O I：
10.1007/s10967-009-7489-3
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Human AFP was used as an antigen for the development of monoclonal antibodies by the hybridoma technique. Balb/c mice were immunized with highly purified AFP. The preparation of I-125-AFP was carried out by lactoperoxidase oxidation method, preparation of AFP standards was carried out from cord sera. The antibody titer of the serum was tested by RIA-AFP system. The spleen of the immunized Balb/c was fused with Sp(2)0 mouse myeloma cells. The cells from the positive hybridomas were cloned twice using limiting dilution method. Eleven stable clones were thus established for secreting monoclonal antibodies to AFP. Cells in this growth phase were chosen for freezing.
引用
收藏
页码：651 / 655
页数：5
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