Targeted gene correction: Synthesis and characterization of double-hairpin 2'-O-methyl RNA/DNA chimera oligonucleotides

被引：2

作者：

Andrus, A

Vinayak, R

Kuimelis, RG

Mullah, B

机构：

来源：

NUCLEOSIDES & NUCLEOTIDES | 1997年 / 16卷 / 7-9期

关键词：

DNA OLIGONUCLEOTIDE; RNA; NUCLEOSIDES; CHEMISTRY; SUPPORT;

D O I：

10.1080/07328319708006155

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of 2'-O-methyl RNA/DNA chimera oligonucleotides were synthesized with a double-hairpin structural motif. Liposome formulated delivery of the chimeras effected targeted, high conversion of mutant alleles in mammalian cell culture. The chimera oligonucleotides were prepared with DNA and 2'-OMe RNA phosphoramidite nucleoside monomers on the ABI 394 synthesizer.

引用

页码：1183 / 1194

页数：12

共 47 条

[41] Synthesis and Multiple Incorporations of 2-O-Methyl-5-hydroxymethylcytidine, 5-Hydroxymethylcytidine and 5-Formylcytidine Monomers into RNA Oligonucleotides
Tanpure, Arun A.
Balasubramanian, Shankar
CHEMBIOCHEM, 2017, 18 (22) : 2236 - 2241
[42] SYNTHESIS AND CHARACTERIZATION OF O-METHYL AND O-ETHYL DITHIOCARBONATE (XANTHATE) DERIVATIVES OF DIMETHYLGERMANE AND DIPHENYLGERMANE - CRYSTAL-STRUCTURE OF PH2GE[S2COME]2
DRAKE, JE
SARKAR, AB
WONG, MLY
INORGANIC CHEMISTRY, 1990, 29 (04) : 785 - 788
[43] ON CHALCOGENOLATES .129. STUDIES ON ESTERS OF N-CYANCARBAMIC ACID .2. SYNTHESIS, SPECTROSCOPIC CHARACTERIZATION, AND DECOMPOSITION OF O-METHYL AND O-ETHYL N-CYANCARBAMATE
HLAWATSCHEK, H
GATTOW, G
ZEITSCHRIFT FUR ANORGANISCHE UND ALLGEMEINE CHEMIE, 1983, 505 (10): : 180 - 186
[44] Synthesis of N3-Methyluridine- and 2′-O-Alkyl/2′-Fluoro-N3-Methyluridine-Modified Phosphoramidites and Their Incorporation into DNA and RNA Oligonucleotides
Sahoo, Avijit
Gore, Kiran R.
CURRENT PROTOCOLS, 2024, 4 (10):
[45] REMARKABLE ENHANCEMENT OF BINDING-AFFINITY OF HETEROCYCLE-MODIFIED DNA TO DNA AND RNA - SYNTHESIS, CHARACTERIZATION AND BIOPHYSICAL EVALUATION OF N-2-IMIDAZOLYLPROPYLGUANINE AND N-2-IMIDAZOLYLPROPYL-2-AMINOADENINE MODIFIED OLIGONUCLEOTIDES
RAMASAMY, KS
ZOUNES, M
GONZALEZ, C
FREIER, SM
LESNIK, EA
CUMMINS, LL
GRIFFEY, RH
MONIA, BP
COOK, PD
TETRAHEDRON LETTERS, 1994, 35 (02) : 215 - 218
[46] Sequence-specific conjugates of oligo(2′-O-methylribonucleotides) and hairpin oligocarboxamide minor-groove binders:: Design, synthesis, and binding studies with double-stranded DNA
Novopashina, DS
Sinyakov, AN
Ryabinin, VA
Venyaminova, AG
Halby, L
Sun, JS
Boutorine, AS
CHEMISTRY & BIODIVERSITY, 2005, 2 (07) : 936 - 952
[47] Synthesis and spectroscopic characterization of new cobalt(III) complexes with 5-methyl-3-formyl pyrazole 3-hexamethyleneiminyl thiosemicarbazone (HMPz3Hex):: X-ray crystallographic identification of HMPz3Hex and [Co(MPz3Hex)2]Br•H2O with evidence for unusual rotation about the azomethine double bond of the ligand on complexation with cobalt(III)
Sau, DK
Butcher, RJ
Chaudhuri, S
Saha, N
POLYHEDRON, 2004, 23 (01) : 5 - 14

← 1 2 3 4 5 →