Cost-effective method for growing three-dimensional cell cultures in extracellular matrix extract

被引：0

作者：

Jacob, A. I. ^{[1
,2
]}

Waite, K. A. ^{[1
,2
,3
]}

Eng, C. ^{[1
,2
,3
,4
,5
]}

机构：

[1] Cleveland Clin Fdn, Genom Med Inst, Cleveland, OH USA

[2] Cleveland Clin Fdn, Lerner Res Inst, Cleveland, OH USA

[3] Cleveland Clin Fdn, Taussig Canc Inst, Cleveland, OH USA

[4] Case Western Reserve Univ, Sch Med, Dept Genet, Cleveland, OH 44106 USA

[5] Case Western Reserve Univ, Sch Med, CASE Comprehens Canc Ctr, Cleveland, OH 44106 USA

来源：

BIOTECHNIC & HISTOCHEMISTRY | 2009年 / 84卷 / 01期

关键词：

co-cultures; EHS; matrigel; three-dimensional; BREAST EPITHELIAL-CELLS; CANCER CELLS; PROLIFERATION; GROWTH; DISRUPTION; COCULTURE; MODELS; BONE;

D O I：

10.1080/10520290802646635

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three-dimensional (3-D) cell cultures are advantageous for modeling complex cellular behavior. A major issue with 3-D cultures grown in Engelbreth-Holm-Swarm (EHS) extracellular matrix extract, however, is the expense. We describe here a simple method using a rubber gasket laid over a coverslip, which provides sufficient 3-D area filled with a minimal amount of EHS, resulting in cost-effective 3-D cellular cultures that are easy to manipulate.

引用

页码：25 / 28

页数：4

共 50 条

[21] IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix
Chen, Kaizhe
Chen, Haoyi
Deng, Lianfu
Yang, Kai
Qi, Jin
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2023, (201):
[22] Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma
Zhang, Yu
Yao, Yupeng
Zhang, Yan
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2019, (146):
[23] Three-dimensional extracellular matrix engineering in the nervous system
Borkenhagen, M
Clémence, JF
Sigrist, H
Aebischer, P
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1998, 40 (03): : 392 - 400
[24] Cost-Effective Three-Dimensional Graphene/Ag Aerogel Composite for High-Performance Sensing
Lou, Xianghong
Zhu, Chengling
Pan, Hui
Ma, Jun
Zhu, Shenmin
Zhang, Di
Jiang, Xueliang
ELECTROCHIMICA ACTA, 2016, 205 : 70 - 76
[25] Generation of Cell-Derived Three Dimensional Extracellular Matrix Substrates from Two Dimensional Endothelial Cell Cultures
Zwolinski, Christopher M.
Ellison, Karen S.
DePaola, Natacha
Thompson, Deanna M.
TISSUE ENGINEERING PART C-METHODS, 2011, 17 (05) : 589 - 595
[26] Cell survival in a three-dimensional matrix
Alavi, Alireza
Stupack, Dwayne G.
INTEGRINS, 2007, 426 : 85 - +
[27] A cost-effective system for facial imaging and three dimensional reconstruction
Shokouhi, SB
Monro, DM
Sherlock, BG
IMAGE DISPLAY - MEDICAL IMAGING 1998, 1998, 3335 : 152 - 161
[28] Tissue origin and extracellular matrix control neutral proteinase activity in human fibroblast three-dimensional cultures
Lorimier, S
Gillery, P
Hornebeck, W
Chastang, F
LaurentMaquin, D
Bouthors, S
Droulle, C
Potron, G
Maquart, FX
JOURNAL OF CELLULAR PHYSIOLOGY, 1996, 168 (01) : 188 - 198
[29] Cartilage-derived extracellular matrix extract promotes chondrocytic phenotype in three-dimensional tissue culture
Youngstrom, Daniel W.
Cakstina, Inese
Jakobsons, Eriks
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY, 2016, 44 (03) : 1040 - 1047
[30] A method to extract three-dimensional objects from three-dimensional NMR image data
Kashiwagi, K
Kose, K
NMR IN BIOMEDICINE, 1997, 10 (01) : 13 - 17

← 1 2 3 4 5 →