Cost-effective method for growing three-dimensional cell cultures in extracellular matrix extract

被引：0

作者：

Jacob, A. I. ^{[1
,2
]}

Waite, K. A. ^{[1
,2
,3
]}

Eng, C. ^{[1
,2
,3
,4
,5
]}

机构：

[1] Cleveland Clin Fdn, Genom Med Inst, Cleveland, OH USA

[2] Cleveland Clin Fdn, Lerner Res Inst, Cleveland, OH USA

[3] Cleveland Clin Fdn, Taussig Canc Inst, Cleveland, OH USA

[4] Case Western Reserve Univ, Sch Med, Dept Genet, Cleveland, OH 44106 USA

[5] Case Western Reserve Univ, Sch Med, CASE Comprehens Canc Ctr, Cleveland, OH 44106 USA

来源：

BIOTECHNIC & HISTOCHEMISTRY | 2009年 / 84卷 / 01期

关键词：

co-cultures; EHS; matrigel; three-dimensional; BREAST EPITHELIAL-CELLS; CANCER CELLS; PROLIFERATION; GROWTH; DISRUPTION; COCULTURE; MODELS; BONE;

D O I：

10.1080/10520290802646635

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Three-dimensional (3-D) cell cultures are advantageous for modeling complex cellular behavior. A major issue with 3-D cultures grown in Engelbreth-Holm-Swarm (EHS) extracellular matrix extract, however, is the expense. We describe here a simple method using a rubber gasket laid over a coverslip, which provides sufficient 3-D area filled with a minimal amount of EHS, resulting in cost-effective 3-D cellular cultures that are easy to manipulate.

引用

页码：25 / 28

页数：4

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