Antifungal activity of melanin in clinical isolates of Candida spp.

被引：4
|
作者：
Fuentes, Marso [1 ]
Hernandez, Romane [1 ]
Gordillo, Diego [1 ]
Amaro, Jose [1 ]
Falconer, Mary A. [1 ]
Alburquenque, Claudio [2 ]
Tapia, Cecilia V. [1 ]
机构：
[1] Univ Chile, Fac Med, Inst Ciencias Biomed, Programa Microbiol & Micol, Santiago, Chile
[2] Univ Mayor, Escuela Tecnol, Santiago, Chile
来源：
REVISTA CHILENA DE INFECTOLOGIA | 2014年 / 31卷 / 01期
关键词：
Melanin; melanocyte; antifungal activity; Candida spp; MELANOCYTES; VIRULENCE; BIOLOGY; CELLS; SKIN;
D O I：
10.4067/S0716-10182014000100004
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Background: Melanocytes are cells located in epidermis and mucous membranes that synthesize melanin and cytokines. It is known that melanin has antimicrobial activity and that melanocytes are melanized in presence of microbial molecules. Objective: To study the antifungal activity of melanin on Candida spp. Methodology: The minimum inhibitory concentration (MIC) to melanin was determined in 4 Candida ATCC strains (C. albicans SC5314, C. parapsilosis 22019, C. glabrata 2001, C. krusei 6258) and 56 clinical isolates of Candida spp. (33 C. albicans, 12 C. glabrata, 3 C. famata, 3 C. krusei, 3 C. parapsilosis, 2 C. tropicalis) using a broth microdilution method. In addition, the antifungal activity of melanocytes and mice melanoma cells was tested against C. albicans. Results: Melanin inhibited the tested isolates, including the susceptible dose-dependent and fluconazole-resistant strains; MIC range and MIC(50)were 0.09-50 mu g/mL and 6.25 mu g/mL, respectively. Pigmented cells lysates inhibited C. albicans. Conclusions: Melanin is able to inhibit clinical isolates of Candida spp. Melanization could be an important protective mechanism of melanocytes.
引用
收藏
页码：28 / 33
页数：6
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