The collagen-containing alginate/poly(L-lysine)/alginate microcapsules

被引：19
|
作者：
Hsu, FY
Tsai, SW
Wang, FF
Wang, YJ [1 ]
机构：
[1] Natl Yang Ming Univ, Inst Biomed Engn, Taipei 112, Taiwan
[2] Natl Yang Ming Univ, Inst Biochem, Taipei 112, Taiwan
来源：
ARTIFICIAL CELLS BLOOD SUBSTITUTES AND IMMOBILIZATION BIOTECHNOLOGY | 2000年 / 28卷 / 02期
关键词：
D O I：
10.3109/10731190009118577
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A method of preparing microcapsules containing collagen fibrous network is reported in this study. This method takes advantage of miscibility of collagen and alginate and the ability of this mixture to form spherical gel beads in the presence of CaCl2. Collagen was then reconstituted within the microcapsules at 37 after alginate was liquefied with citrate. GH3 rat pituitary tumor cell, which can be cultured. in both suspended and attached forms, were entrapped within the microcapsules. The cell proliferated faster in the collagen-containing capsule as compared to those in the conventional microcapsules.
引用
收藏
页码：147 / 154
页数：8
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