Multidimensional single-molecule imaging in live cells using total-internal-reflection fluorescence microscopy

被引：30
|
作者：
Webb, S. E. D. [1 ]
Needham, S. R. [1 ]
Roberts, S. K. [1 ]
Martin-Fernandez, M. L. [1 ]
机构：
[1] CCLRC, Daresbury Lab, Warrington WA4 4AD, Cheshire, England
来源：
OPTICS LETTERS | 2006年 / 31卷 / 14期
基金：
英国生物技术与生命科学研究理事会;
关键词：
D O I：
10.1364/OL.31.002157
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
We have developed a wide-field total-internal-reflection fluorescence microscope capable of imaging single molecules in live cells, resolved in both wavelength and polarization. We show fluorescence resonance energy transfer between single pairs of fluorescent molecules bound to signaling receptors in the plasma membrane of live cells and demonstrate the importance of polarization discrimination in addition to wavelength separation. (c) 2006 Optical Society of America.
引用
收藏
页码：2157 / 2159
页数：3
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