Defective NF-kB Transcription Factor as the Mediator of Inflammatory Responses: A Study on Depressed Iranian Medical Students

被引:20
|
作者
Nazari, Mahmood [1 ]
Khodadadi, Hassan [2 ]
Fathalizadeh, Javad [3 ]
Hassanshahi, Gholamhossein [4 ]
Bidaki, Reza [5 ]
Ayoobi, Fatemeh [6 ]
Hajebrahimi, Batool [7 ]
Bagheri, Fatemeh [3 ]
Arababadi, Mohammad Kazemi [3 ]
机构
[1] Rafsanjan Univ Med Sci, Fac Med, Dept Biochem, Rafsanjan, Iran
[2] Rafsanjan Univ Med Sci, Fac Nursing, Dept Nursing, Rafsanjan, Iran
[3] Rafsanjan Univ Med Sci, Immunol Infect Dis Res Ctr, Rafsanjan, Iran
[4] Rafsanjan Univ Med Sci, Mol Med Res Ctr, Rafsanjan, Iran
[5] Rafsanjan Univ Med Sci, Dept Psychiat, Rafsanjan, Iran
[6] Rafsanjan Univ Med Sci, Physiol Pharmacol Res Ctr, Rafsanjan, Iran
[7] Shahid Bahonar Univ, Fac Psychol, Dept Psychol, Kerman, Iran
关键词
depression; NF-kB; Real-Time PCR; ACTIVATION; MYD88;
D O I
10.7754/Clin.Lab.2012.120809
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: NF-kB is a transcription factor that is a downstream target of several cell signaling systems including TLRs. Defective expression of the molecule can lead to inappropriate immune responses. Previous studies revealed that depression can affect immune responses, but its molecular mechanisms are yet to be fully understood. Thus, the main aim of this study was to identify if mRNA levels of NF-kB are changed in the PBMCs isolated from Iranian depressed medical students. Methods: This cross-sectional study was done on 38 Iranian depressed medical students and 43 healthy students as a control group. The mRNA levels of NF-kB were assessed in parallel with beta-actin (as the housekeeping gene) using Real-Time PCR technique. Results: Our results showed that mRNA levels of NF-kB were significantly decreased in isolated PBMCs from depressed patients compared to healthy controls. Conclusions: According to the results obtained in the present study, it seems that depressed patients are unable to appropriately express NF-kB at mRNA levels which may in turn lead to defective molecule expression.
引用
收藏
页码:827 / 830
页数:4
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