Selection of synonymous codons for better expression of recombinant proteins in tobacco chloroplasts

被引:2
|
作者
Nakamura, Masayuki [1 ]
Sugiura, Masahiro [1 ,2 ]
机构
[1] Nagoya City Univ, Grad Sch Nat Sci, Aichi 4678501, Japan
[2] Sugiyama Jogakuen Univ, Sugiyama Human Res Ctr, Aichi 4648662, Japan
关键词
Chloroplast; codon usage; synonymous codon; tobacco; translation efficiency; TRANSFER-RNA GENES; PLASTID TRANSFORMATION; TRANSLATION; COMPILATION; SEQUENCE; TRANSCRIPTS;
D O I
10.5511/plantbiotechnology.26.53
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The 20 amino acids, except for methionine and tryptophan, are coded for by two to six codons called synonymous codons. Synonymous codons are used differently by different organism. Hence, codon selection should be important to express recombinant proteins in tobacco chloroplasts. We present here the codon usage table from the entire 79 mRNAs from tobacco chloroplasts. As codons function on mRNAs, codon usage tables should be constructed from mRNAs but not from genes. We devised an in vitro assay for translation efficiencies of codons, and measured the translation efficiencies of several synonymous codon groups in tobacco chloroplasts. Our results indicated that translation efficiencies of individual codons are not always correlated with codon usage. Based on these data, we discuss on synonymous codon selection for expression of inserted genes in tobacco chloroplasts.
引用
收藏
页码:53 / 56
页数:4
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