Immobilization of Fibrinogen Antibody on Self-Assembled Gold Monolayers for Immunosensor Applications

被引：6
|
作者：
Cho, Hongsik [1 ,2 ,3 ]
Zook, Justin [4 ]
Banner, Todd
Park, Sang-Hyug [5 ]
Min, Byoung-Hyun [6 ,7 ,8 ]
Hasty, Karen A. [1 ,2 ,3 ]
Pinkhassik, Eugene
Lindner, Erno [4 ]
机构：
[1] Univ Tennessee, Hlth Sci Ctr, Dept Orthpaed Surg & Biomed Engn, Memphis, TN 38104 USA
[2] Campbell Clinn, Dept Orthopaed Surg, Memphis, TN 38104 USA
[3] Vet Affairs Med Ctr, Memphis, TN 38104 USA
[4] Univ Memphis, Dept Biomed Engn, Memphis, TN 38152 USA
[5] Jungwon Univ, Dept Biomed Engn, Geosan 367700, South Korea
[6] Ajou Univ, Sch Med, Dept Orthopead Surg, Suwon 443749, South Korea
[7] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea
[8] Ajou Univ, Med Ctr, Cell Therapy Ctr, Suwon 443749, South Korea
来源：
TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2014年 / 11卷 / 01期
基金：
美国国家科学基金会;
关键词：
self-assembled monolayers (SAMs); anti-fibrinogen; immunosensors; enzyme-linked immuno sorbent assay (ELISA); ellipsometry; atomic force microscopy (AFM); gold monolayer; antibody immobilization; BIOSENSING APPLICATIONS; PLASMA-FIBRINOGEN; SURFACE; NANOTECHNOLOGY; PROTEINS;
D O I：
10.1007/s13770-013-1119-4
中图分类号：
Q813 [细胞工程];
学科分类号：
摘要：
Self-assembled gold monolayers offer several advantages for the realization of novel modified electrodes for biosensor applications. This is due to their ability to decrease non-specific adsorption and provide for covalent attachment of biomolecules. Surfaces for these applications require the precise control of ligand density, the ability to immobilize ligands, and in situ-modulation of ligand activity. In this study, we focused our studies on the immobilization of antibody on a gold monolayer surface. We self-assembled thioctic acid onto the gold surface as an anchor point for the immobilization of anti-fibrinogen onto the surface. The modifications to the gold surface were characterized by ELISA, ellipsometry, and AFM.
引用
收藏
页码：10 / 15
页数：6
相关论文
共 50 条
  • [1] Immobilization of fibrinogen antibody on self-assembled gold monolayers for immunosensor applications
    Hongsik Cho
    Justin Zook
    Todd Banner
    Sang-Hyug Park
    Byoung-Hyun Min
    Karen A. Hasty
    Eugene Pinkhassik
    Erno Lindner
    Tissue Engineering and Regenerative Medicine, 2014, 11 : 10 - 15
  • [2] A MEMS Based Amperometric Immunosensor with Self-assembled Monolayers Immobilization Technique
    Yuanyuan Xu Chao Bian Shaofeng Chen Shanhong Xia State Key Laboratory of Transducer TechnologyInstitute of ElectronicsChinese Academy of SciencesBeijing China
    稀有金属材料与工程, 2006, (S3) : 344 - 345
  • [3] A MEMS based amperometric immunosensor with self-assembled monolayers immobilization technique
    Xu, Yuanyuan
    Bian, Chao
    Chen, Shaofeng
    Xia, Shanhong
    RARE METAL MATERIALS AND ENGINEERING, 2006, 35 : 344 - 345
  • [4] Immobilization of methylene blue on self-assembled iodine monolayers on gold
    Vela, Maria E.
    Vericat, C.
    Millone, M. A. Daza
    Fonticelli, M.
    Benitez, G.
    Salvarezza, Roberto C.
    Tognalli, Nicolas G.
    Fainstein, Alejandro
    JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2006, 6 (08) : 2362 - 2367
  • [5] Immobilization of DNA through intercalation at self-assembled monolayers on gold
    Higashi, N
    Takahashi, M
    Niwa, M
    LANGMUIR, 1999, 15 (01) : 111 - 115
  • [6] Immobilization of antibody on self-assembled monolayers of ω-carboxylated thiols.
    Rhim, G
    Yoon, M
    Park, U
    Han, HS
    Kim, JH
    Hwang, YJ
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2000, 219 : U94 - U94
  • [7] Applications of self-assembled monolayers (SAMs) of alkanethiolates on gold
    Tam-Chang, SW
    Iverson, I
    ADSORPTION AND ITS APPLICATIONS IN INDUSTRY AND ENVIRONMENTAL PROTECTION, VOL I: APPLICATIONS IN INDUSTRY, 1999, 120 : 917 - 950
  • [8] Immobilization of proteins on self-assembled monolayers
    Wadu-Mesthrige, K
    Amro, NA
    Liu, GY
    SCANNING, 2000, 22 (06) : 380 - 388
  • [9] Oriented antibody immobilization on self-assembled monolayers applied as impedance biosensors
    Tsugimura, Kaiki
    Ohnuki, Hitoshi
    Wu, Haiyun
    Endo, Hideaki
    Tsuya, Daiju
    Izumi, Mitsuru
    12TH INTERNATIONAL CONFERENCE ON NANOMOLECULAR ELECTRONICS (ICNME-2016), 2017, 924
  • [10] STABILITY OF SELF-ASSEMBLED MONOLAYERS ON GOLD FOR MEMS/NEMS APPLICATIONS
    Ang, X. F.
    Lin, A. T.
    Li, J.
    Wei, J.
    Chen, Z.
    Wong, C. C.
    IMECE2008: PROCEEDINGS OF THE INTERNATIONAL MECHANICAL ENGINEERING CONGRESS AND EXPOSITION - 2008, VOL 6, 2009, : 5 - 10
12345