A MEMS Based Amperometric Immunosensor with Self-assembled Monolayers Immobilization Technique

被引:0
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作者
Yuanyuan Xu Chao Bian Shaofeng Chen Shanhong Xia State Key Laboratory of Transducer TechnologyInstitute of ElectronicsChinese Academy of SciencesBeijing China [100080 ]
机构
关键词
amperometric immunosensor; microelectromechanical systems; self-assembled monolayers; gold nanoparticles; protein G; human immunoglobulin;
D O I
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中图分类号
TP212 [发送器(变换器)、传感器];
学科分类号
080202 ;
摘要
<正>Based on MEMS technology,immunosensor with an"Au,Pt,Pt"three-microelectrode system enclosed in a SU-8 micro pool was fabricated.Employing SAMs technique,the Au electrode was modified by cysteamine(Cys)to assemble gold nanopanicles(nanogold)layer,subsequently,a layer of protein G(PG)was immobilized on nanogold layer to further capture antibody orientedly.Compared with the immunosensors using bulky gold electrode and direct PG binding to electrode immobilization technique for antibody,it has attractive advantages,such as miniaturization,good compatibility,broad linear range for human immunoglobulin(HIgG)and easy to be designed into array.
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页码:344 / 345
页数:2
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