Biochemical Properties of a Novel d-Mannose Isomerase from Pseudomonas syringae for d-Mannose Production

被引：7

作者：

Hua, Xiaohan ^{[1
]}

Li, Yanxiao ^{[2
]}

Jiang, Zhengqiang ^{[1
]}

Ma, Junwen ^{[2
]}

Liu, Haijie ^{[1
]}

Yan, Qiaojuan ^{[2
]}

机构：

[1] China Agr Univ, Key Lab Food Bioengn China Natl Light Ind, Coll Food Sci & Nutr Engn, 17 Qinghua Donglu, Beijing 100083, Peoples R China

[2] China Agr Univ, Beijing Adv Innovat Ctr Food Nutr & Human Hlth, Coll Engn, 17 Qinghua Donglu, Beijing 100083, Peoples R China

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2021年 / 193卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Mannose; Mannose isomerase; Characterization; Pseudomonas syringae; Mannose production;

D O I：

10.1007/s12010-021-03487-y

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

d-Mannose isomerase can reversibly catalyze d-fructose to d-mannose which has various beneficial effects. A novel d-mannose isomerase gene (PsMIaseA) from Pseudomonas syringae was cloned and expressed in Escherichia coli. The recombinant d-mannose isomerase (PsMIaseA) showed the highest amino acid sequence homogeneity of 50% with ManI from Thermobifda fusca. PsMIaseA was purified through Ni-NTA chromatography, and its specific activity was 818.6 U mg(-1). The optimal pH and temperature of PsMIaseA were pH 7.5 and 45 degrees C, respectively. The enzyme was stable within a wide pH range from 5.0 to 10.0. It could efficiently convert d-fructose to d-mannose without any metal ions. When PsMIaseA was incubated with 600 g/L d-fructose for 6 h, the space-time yield of d-mannose reached 27.2 g L-1 h(-1) with a maximum conversion ratio of 27%. Therefore, the d-mannose isomerase may be suitable for green production of d-mannose.

引用

页码：1482 / 1495

页数：14

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