Determination of acid phosphatase activity in a double injection flow system with electrochemical detection

被引：8

作者：

Castanón-Fernández, J ^{[1
]}

Fernández-Abedul, MT ^{[1
]}

Costa-García, A ^{[1
]}

机构：

[1] Univ Oviedo, Dept Quim Fis & Analit, Asturias, Spain

来源：

ANALYTICA CHIMICA ACTA | 2000年 / 406卷 / 02期

关键词：

acid phosphatase; double-injection; stopped-flow; FIA-ECD;

D O I：

10.1016/S0003-2670(99)00772-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An enzyme assay for the measurement of total and prostatic acid phosphatase activity by flow injection analysis with electrochemical detection has been developed. The substrate (alpha-naphthyl phosphate) and enzyme are simultaneously injected to the system by means of a double injection valve. The alpha-naphtol produced after stopping the flow is amperometrically quantified. The calibration curve is linear from 0.01 to 1.3 u/l with a limit of detection of 0.006 u/l. Results were compared with that obtained spectrophotometrically. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：225 / 232

页数：8

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