The relationship between ST6Gal I Golgi retention and its cleavage-secretion

被引:0
|
作者
Kitazume-Kawaguchi, S
Dohmae, N
Takio, K
Tsuji, S
Colley, KJ
机构
[1] Univ Illinois, Dept Biochem & Mol Biol, Coll Med, Chicago, IL 60612 USA
[2] RIKEN, Inst Phys & Chem Res, Frontier Res Program, Dept Biomol Characterizat, Wako, Saitama 3510198, Japan
关键词
glycosyltransferase; Golgi; secretion; sialyltransferase;
D O I
暂无
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ST6Gal I is a sialyltransferase that modifies N-linked oligosaccharides of glycoproteins. Previous results suggested a role for luminal stem and active domain sequences in the efficiency of ST6Gal I Golgi retention. Characterization of a series of STtyr isoform deletion mutants demonstrated that the stem is sensitive to proteases and that preventing cleavage in this region leads to increased cell surface expression, A mutant lacking amino acids 32-104 (ST Delta 4) is not active or cleaved and secreted like the wild type STtyr, but does exhibit increased cell surface expression. It is probable that the ST Delta 4 mutant lacks the stem region and some amino acids of the active domain because the ST Delta 5 mutant lacking amino acids 86-104 is also not active but is cleaved and secreted. In contrast, deletion of stem amino acids between residues 32 and 86 in the ST Delta 1, ST Delta 2, and ST Delta 3 mutants does not inactive these enzyme forms, eliminate their cleavage and secretion, or increase their cell surface expression. Surprisingly, cleavage occurs even though the previously identified Asn63-Ser 64 cleavage site is missing, Further evaluation demonstrated that a cleavage site between Lys 40 and Glu 41 is used in COS cells. Mutagenesis of Lys 40 significantly decreased, but did not eliminate cleavage, suggesting that there are additional secondary sites of cleavage in the ST6Gal I stem.
引用
收藏
页码:1397 / 1406
页数:10
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