Rapid Determination of Amino Acids in Ginseng by High Performance Liquid Chromatography and Chemometric Resolution

被引:3
|
作者
Han Eng [1 ]
Wu Xi [1 ]
Cai Wensheng [1 ]
Shao Xueguang [1 ]
机构
[1] Nankai Univ, Coll Chem, Res Ctr Analyt Sci, Collaborat Innovat Ctr Chem Sci & Engn Tianjin,St, Tianjin 300071, Peoples R China
基金
中国国家自然科学基金;
关键词
High performance liquid chromatography(HPLC); Chemometrics; Amino acid; Ginseng; Immune algorithm; TRILINEAR DECOMPOSITION ALGORITHM; EVOLVING LATENT PROJECTIONS; 2-WAY MULTICOMPONENT DATA; 2ND-ORDER CALIBRATION; CURVE RESOLUTION; HPLC-DAD; DERIVATIZATION; CONSTITUENTS; COMPONENTS; SEPARATION;
D O I
10.1007/s40242-014-3543-y
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Ginseng is one of the most important traditional Chinese medicines and functional foods. A method for the fast determination of amino acids in ginseng samples using high performance liquid chromatography(HPLC) was developed, in which strong isocratic elution was employed for simplifying the separation and speeding up the analysis. All amino acids were eluted within 3 min with the chromatogram composed of overlapped peaks from the interferences. Then, non-negative immune algorithm(NNIA) was adopted to resolve the chromatographic signals of the components from the chromatogram measured. The results show that the signals of the amino acids can be correctly extracted by NNIA and the signal extracted can be used for the quantitative analysis. The method was validated via determining six amino acids of four different samples of ginseng. The recoveries of the spiked samples are in a range of 96.6%-106.3%.
引用
收藏
页码:578 / 581
页数:4
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