The complete mitochondrial genome of the scuttle fly, Megaselia scalaris (Diptera: Phoridae)

被引:19
|
作者
Zhong, Ming [1 ]
Wang, Xiang [2 ]
Liu, Qinlai [3 ]
Luo, Baihua [1 ]
Wu, Chang [1 ]
Wen, Jifang [1 ]
机构
[1] Cent S Univ, Sch Basic Med Sci, Dept Pathol, Changsha 410013, Hunan, Peoples R China
[2] Dalian Med Univ, Ctr Canc, Inst Canc Stem Cell, Dalian, Liaoning, Peoples R China
[3] Taishan Med Univ, Dept Pathol, Tai An, Shandong, Peoples R China
关键词
A plus T-rich region; Forensic myiasis; species identification; tRNA; PCR-RFLP; MYIASIS; IDENTIFICATION; CALLIPHORIDAE; SARCOPHAGIDAE; SEQUENCE;
D O I
10.3109/19401736.2013.879651
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
More than 1400 scuttle flies species in worldwide comprise the Megaselia genus, the largest genus in the family Phoridae. The complete mitochondrial genome of Megaselia scalaris, a medically important entomology was sequenced for the first time. The 15,599bp circular genome contains the 37 genes found in a typical Metazoan genome: 13 protein-coding genes, 22 transfer RNA genes and 2 ribosomal RNA genes. The mitochondrial genome also contains one non-coding A+T-rich region. The arrangement of the genes was identical with other insect. Each of the base composition on heavy strand was as follows A: 38.87%, G: 13.74%, C: 9.46%, T: 37.93% and the A+T content 76.80%. The mitochondrial genome of M. scalaris presented may be valuable for determining phylogenetic relationships within the order Diptera and especially for the family Phoridae. These sequences could also be used to select reliable molecular markers for species identification in forensic entomology.
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页码:182 / 184
页数:3
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