Process of thermal destruction of glucose in consequence of sterilization in autoclave in the biopharmaceutical manufacturing

The results of studying the process of glucose' thermal decomposition in consequence of the sterilization in the autoclave and the shelf life are considered. The research of the process was carried out for 5% glucose solution for injections before and after the sterilization in the autoclave by saturated steam at 110 degrees C for 30 minutes by the study of UV absorption spectra, granulometric analysis in the interval 0.2-25.0 mkm and pH measurements. The spectra of the glucose solutions before the sterilization were found to have no absorption bands in the range from 200nm to 400nm After the sterilization two absorption bands with maximal at 229nm and 284nm respectively appeared. After three-month storage only one absorption band with maximum at 284nm remains. In the report the observed spectral effects are explained.