Purification and characterization of the Escherichia coli thermoresistant gluconokinase encoded by the gntK gene

被引：16

作者：

Izu, H ^{[1
]}

Adachi, O ^{[1
]}

Yamada, M ^{[1
]}

机构：

[1] YAMAGUCHI UNIV,FAC AGR,DEPT BIOL CHEM,YAMAGUCHI 753,JAPAN

来源：

FEBS LETTERS | 1996年 / 394卷 / 01期

关键词：

gluconokinase; gntK gene; gntV gene;

D O I：

10.1016/0014-5793(96)00923-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A thermoresistant gluconokinase encoded by the gutK gene of Escherichia coli K-12 was purified and characterized. The K-m values of the purified enzyme for gluconate and ATP are 42 mu M and 123 mu M, respectively, and the activity was not altered by the presence of pyruvate, The enzyme was shown to function as a dimer with two identical subunits of 18.4 kDa. These characteristics appear to be distinct from those of the gluconokinase reported by E.I. Vivas, A, Liendo, K. Dawidowicz, and T. Isturiz (1994) J. Basic, Microbiol. 16, 117-122.

引用

页码：14 / 16

页数：3

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