A beta-glucosidase gene downstream of the cellulose synthase operon in cellulose-producing Acetobacter

被引:21
|
作者
Tonouchi, N [1 ]
Tahara, N [1 ]
Kojima, Y [1 ]
Nakai, T [1 ]
Sakai, F [1 ]
Hayashi, T [1 ]
Tsuchida, T [1 ]
Yoshinaga, F [1 ]
机构
[1] KYOTO UNIV, WOOD RES INST, UJI, KYOTO 611, JAPAN
关键词
Acetobacter; cellulose synthesis; beta-glucosidase; gene;
D O I
10.1271/bbb.61.1789
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
An open reading frame was found 214 bp downstream of the cellulose synthase operon of Acetobacter. The encoded amino acid sequence was found to be similar to some beta-glucosidases (G3ases), We detected G3ase activity in the culture medium and analysis of the N-terminal amino acid sequence showed that this gene encodes the enzyme, Therefore, it is possible that this region is a gene cluster for cellulose synthesis.
引用
收藏
页码:1789 / 1790
页数:2
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