Comparison of 'real-time' and immunometric RT-PCR: RNA interference of reverse transcriptase-PCR

被引：1
|
作者：
Hoadley, ME [1 ]
Hopkins, SJ [1 ]
机构：
[1] Univ Manchester, Hope Hosp, Div Med & Neurosci, Salford M6 8HD, Lancs, England
来源：
JOURNAL OF IMMUNOLOGICAL METHODS | 2006年 / 312卷 / 1-2期
关键词：
RT-PCR; mRNA; 'real-time';
D O I：
10.1016/j.jim.2006.02.012
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We measured interleukin-6 (IL-6) mRNA in whole blood, using an immunometric reverse transcriptase-polymerase chain reaction (IRT-PCR) method and 'real-time' RT-PCR using the Roche LightCycler. Both methods showed similar precision, in terms of inter- and intra-assay variation, where a lower total RNA concentration was used at a relatively high ratio of (IL-6) mRNA to total RNA. However, the 'real-time' RT-PCR method showed a greater loss of accuracy at higher concentrations of total RNA. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码：40 / 44
页数：5
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