Biocompatibility and Bioactivity of Set Direct Pulp Capping Materials on Human Dental Pulp Stem Cells

被引:29
|
作者
Kim, Yemi [1 ]
Lee, Donghee [2 ]
Song, Dani [3 ]
Kim, Hye-Min [3 ]
Kim, Sin-Young [3 ]
机构
[1] Ewha Womans Univ, Sch Med, Dent Res Inst, Dept Conservat Dent, Seoul 07985, South Korea
[2] Catholic Univ Korea, Coll Med, Seoul 06591, South Korea
[3] Catholic Univ Korea, Seoul St Marys Hosp, Dept Conservat Dent, Coll Med, 222 Banpo Daero, Seoul 06591, South Korea
基金
新加坡国家研究基金会;
关键词
cell viability; cell migration; pulp capping materials; ALP activity; ARS assay; MINERAL TRIOXIDE AGGREGATE; CALCIUM HYDROXIDE; IN-VITRO; PROGNOSTIC-FACTORS; BIODENTINE; CYTOTOXICITY; MTA; RESPONSES; CEMENTS; TEETH;
D O I
10.3390/ma13183925
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
In this study, we assessed the biocompatibility and bioactivity of various pulp capping materials-ProRoot MTA (Dentsply Tulsa Dental Specialties), Biodentine (Septodont), TheraCal LC (Bisco), and Dycal (Dentsply Caulk)-on human dental pulp stem cells (hDPSCs). Experimental disks (diameter, 7 mm; height, 4 mm) were stored in a humified incubator at 37 degrees C for 48 h. Then, the pulp capping materials were tested for cytotoxic effects by methyl-thiazoldiphenyl-tetrazolium and scratch wound healing assays, and for mineralization potential by Alizarin red S (ARS) staining assay and alkaline phosphatase enzyme (ALP) activity. Cell viability and cell migration did not significantly differ between ProRoot MTA, Biodentine, and control (p > 0.05). TheraCal LC exhibited slower cell migration on days 2-4 compared to control (p 0.05), and Dycal showed no cell migration. ALP activity was highest with Biodentine on days 10 and 14, and was lowered with TheraCal LC and Dycal (p < 0.05). In the ARS assay, hDPSCs grown in ProRoot MTA and TheraCal LC eluates showed significantly increased mineralized nodule formation on day 21 compared to Biodentine, Dycal, and control (p < 0.05). These findings indicate that ProRoot MTA, Biodentine, and TheraCal LC exhibit better biocompatibility and bioactivity than Dycal.
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页数:11
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