New -Casein Alleles in Jakhrana Goat Affecting Milk Processing Properties

High genetic variability in the -Casein gene that can be exploited for the marker assisted selection milk production of desirable processing and nutritional properties. We resolved seven unique PCR-SSCP variants in a 425 bp segment of -Casein exon-4 (CSN*3) on PAGE gel and then sequenced the amplicons. All SSCP haplotypes revealed novel sequences (NCBI Genbank accessions EF053350- EF053356). Nucleotide substitutions at nucleotide positions 245, 247, 274, 345 and 471 caused single nucleotide polymorphism (SNP). We identified seven new alleles CSN3*N, CSN3*O, CSN3*P, CSN3*Q and CSN3*R being homozygous and CSN3*AH, CSN3*KH being heterozygous types. In silico analysis yielded an isoelectric point of 5.29 and 5.63 for the conceptual proteins based on CSN3*AH and CSN3*KH, CSN3*N, CSN3*O, CSN3*P, CSN3*Q, CSN3*R allele sequence, respectively, which were of IEFA and IEFB type -Casein (CSN3).